Hotspot

trnR-UCU-trnG-UCC

LSC · 30002-30219 · 218 bp

Back to Dolichoglottis

Pi 0.0293
MarkerSeek score 72.00
Primer available yes
Label trnR-UCU-trnG-UCC

Feature Metadata

label_name
trnR-UCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
218
variable_sites
6
pi
0.0293
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-trnG-UCC_p1 AGAAGACCTCTGTCCTATCCA AGCCTTCCAAGCTAACGATG 47.6 50.0 56.9 58.0 258-298 bp 1.0 67.4
trnR-UCU-trnG-UCC_p2 AGAAGACCTCTGTCCTATCCA CCTAGCCTTCCAAGCTAACG 47.6 55.0 56.9 58.1 258-301 bp 1.0 67.1
trnR-UCU-trnG-UCC_p3 AGGTTTAGAAGACCTCTGTCCT AGCCTTCCAAGCTAACGATG 45.5 50.0 57.9 58.0 264-304 bp 1.0 66.6
trnR-UCU-trnG-UCC_p4 AGGTTTAGAAGACCTCTGTCCT CCTAGCCTTCCAAGCTAACG 45.5 55.0 57.9 58.1 264-307 bp 1.0 66.4
trnR-UCU-trnG-UCC_p5 AGAAGACCTCTGTCCTATCCA CCCTAGCCTTCCAAGCTAAC 47.6 55.0 56.9 57.7 258-302 bp 1.0 64.9