Hotspot

atpI-atpH

LSC · 25222-26313 · 1092 bp

Back to Dolichoglottis

Pi 0.000927
MarkerSeek score 45.60
Primer available yes
Label atpI-atpH

Feature Metadata

label_name
atpI-atpH
feature_type
igs
region
LSC
length_bp
1092
variable_sites
1
pi
0.000927
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-atpH_p1 TGCTTTGGCTTTACTCACGT TTATTTTTCCCTCCGCCTCG 45.0 50.0 58.0 58.0 1571-1574 bp 1.0 79.9
atpI-atpH_p2 TGCTTTGGCTTTACTCACGT CTCGCGATACCTTCTACAGC 45.0 55.0 58.0 58.0 1545-1548 bp 1.0 79.8
atpI-atpH_p3 TGCTTTGGCTTTACTCACGT TGGCCCAAAGAAACGAAAGA 45.0 45.0 58.0 57.9 2012-2102 bp 1.0 79.6
atpI-atpH_p4 TTCGGAACCCACAAACCATT TTATTTTTCCCTCCGCCTCG 45.0 50.0 57.9 58.0 1812-1815 bp 1.0 79.5
atpI-atpH_p5 AAATCGGGGGTTTCCAAGTC TTATTTTTCCCTCCGCCTCG 50.0 50.0 58.1 58.0 1899-1902 bp 1.0 79.5