Hotspot

trnS-GCU-trnC-GCA

LSC · 8660-9400 · 741 bp

Back to Dolichoglottis

Pi 0.00271

MarkerSeek score 60.20

Primer available yes

Label trnS-GCU-trnC-GCA

Feature Metadata

label_name: trnS-GCU-trnC-GCA
feature_type: igs
region: LSC
length_bp: 741
variable_sites: 2
pi: 0.00271
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnC-GCA_p1	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	892-894 bp	1.0	78.3
trnS-GCU-trnC-GCA_p2	ACGGAAAGAGAGGGATTCGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	58.4	874-876 bp	1.0	76.1
trnS-GCU-trnC-GCA_p3	ACGGAAAGAGAGGGATTCGA	GTTGATTGTTGATGAGGCGG	50.0	50.0	58.2	57.5	915-917 bp	1.0	74.6
trnS-GCU-trnC-GCA_p4	CTTTCGTCCACTCAGCCATC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.6	58.1	828-830 bp	1.0	74.4
trnS-GCU-trnC-GCA_p5	AACGGAAAGAGAGGGATTCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.3	58.1	893-895 bp	1.0	74.1