Hotspot

rps16-trnQ-UUG

LSC · 6329-7296 · 968 bp

Back to Dolichoglottis

Pi 0.00519

MarkerSeek score 62.00

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 968
variable_sites: 5
pi: 0.00519
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1041-2835 bp	1.0	78.6
rps16-trnQ-UUG_p2	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1053-1056 bp	1.0	77.0
rps16-trnQ-UUG_p3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1052-1055 bp	1.0	77.0
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1045-2839 bp	1.0	73.9
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	GGTAAGGCAACGGGTTTTGA	45.0	50.0	58.1	58.7	1073-2867 bp	1.0	73.8