Hotspot

rps12-rps7

IRa · 145940-145992 · 53 bp

Back to Dobinea

Pi 0.0189

MarkerSeek score 63.30

Primer available yes

Label rps12-rps7

Feature Metadata

label_name: rps12-rps7
feature_type: igs
region: IRa
length_bp: 53
variable_sites: 1
pi: 0.0189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rps7_p1	CTGTGGAAAGCCGTATTCGA	TGTAGTACCTCGACGTGACA	50.0	50.0	58.0	57.8	179 bp	1.0	79.1
rps12-rps7_p2	TCTGTGGAAAGCCGTATTCG	TGTAGTACCTCGACGTGACA	50.0	50.0	58.0	57.8	180 bp	1.0	79.1
rps12-rps7_p3	GGCTTGGAGGGAGATCTTTC	TGTAGTACCTCGACGTGACA	55.0	50.0	57.7	57.8	143 bp	1.0	76.5
rps12-rps7_p4	AGAATTCTGTGGAAAGCCGT	TGTAGTACCTCGACGTGACA	45.0	50.0	57.4	57.8	185 bp	1.0	74.5
rps12-rps7_p5	CTGTGGAAAGCCGTATTCGA	TCTGTAGTACCTCGACGTGA	50.0	50.0	58.0	57.3	181 bp	1.0	74.4