Hotspot

ndhI-ndhA

SSC · 124542-124622 · 81 bp

Back to Dobinea

Pi 0.0247

MarkerSeek score 63.30

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 81
variable_sites: 2
pi: 0.0247
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	CCCGTACTGTTTGTTGACCA	CTTCCCAACTCCTTTCACTGT	50.0	47.6	58.0	57.8	156 bp	1.0	71.0
ndhI-ndhA_p2	CCCGTACTGTTTGTTGACCA	TTCCCAACTCCTTTCACTGT	50.0	45.0	58.0	56.6	155 bp	1.0	69.3
ndhI-ndhA_p3	CCCGTACTGTTTGTTGACCA	TCTTCCCAACTCCTTTCACTG	50.0	47.6	58.0	57.5	157 bp	1.0	68.7
ndhI-ndhA_p4	AGCCCGTACTGTTTGTTGAC	CTTCCCAACTCCTTTCACTGT	50.0	47.6	58.4	57.8	158 bp	1.0	68.0
ndhI-ndhA_p5	CAGCCCGTACTGTTTGTTGA	CTTCCCAACTCCTTTCACTGT	50.0	47.6	58.4	57.8	159 bp	1.0	68.0