Hotspot

rpl32-trnL-UAG

SSC · 117826-118896 · 1071 bp

Back to Dobinea

Pi 0.0132

MarkerSeek score 66.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1071
variable_sites: 14
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGCAGCGTTGAAAGCTTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.4	58.0	1171-1185 bp	1.0	78.6
rpl32-trnL-UAG_p2	GGCAGCGTTGAAAGCTTTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.4	58.0	1172-1186 bp	1.0	78.6
rpl32-trnL-UAG_p3	AAAAGGGGGATATTGGGCAG	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.5	58.0	1186-1200 bp	1.0	78.2
rpl32-trnL-UAG_p4	AAAAGGGGGATATTGGGCAG	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.5	58.0	1187-1201 bp	1.0	78.2
rpl32-trnL-UAG_p5	GGCAGCGTTGAAAGCTTTTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.4	57.9	1181-1195 bp	1.0	77.7