Hotspot

ndhC-trnV-UAC

LSC · 53745-54369 · 625 bp

Back to Dobinea

Pi 0.0096
MarkerSeek score 62.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
625
variable_sites
6
pi
0.0096
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TAATCGGGGCTAAAACTGCG AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.3 57.8 761-1057 bp 1.0 76.2
ndhC-trnV-UAC_p2 CCTTTGCTAATCGGGGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 768-1064 bp 1.0 76.2
ndhC-trnV-UAC_p3 CTTCTCTGGCCCTTTGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.5 57.8 778-1074 bp 1.0 75.0
ndhC-trnV-UAC_p4 TAATCGGGGCTAAAACTGCG GAAGGTCTACGGTTCGAGTC 50.0 55.0 58.3 57.5 760-1056 bp 1.0 73.5
ndhC-trnV-UAC_p5 CCTTTGCTAATCGGGGCTAA GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.7 57.5 767-1063 bp 1.0 73.5