Hotspot

trnG-GCC-trnfM-CAU

LSC · 39194-39354 · 161 bp

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Pi 0.0187

MarkerSeek score 63.90

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 161
variable_sites: 3
pi: 0.0187
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	238-239 bp	1.0	79.7
trnG-GCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	CTTTTCTTCATCGCGGGGTA	50.0	50.0	57.8	58.0	289-290 bp	1.0	79.6
trnG-GCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	TTTTCTTCATCGCGGGGTAG	50.0	50.0	57.8	58.0	288-289 bp	1.0	79.6
trnG-GCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.8	58.0	274-275 bp	1.0	79.6
trnG-GCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.8	57.8	236-237 bp	1.0	78.6