Hotspot

psbM-trnD-GUC

LSC · 31455-32523 · 1069 bp

Back to Dobinea

Pi 0.0131

MarkerSeek score 63.30

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1069
variable_sites: 14
pi: 0.0131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	TGTCCCGGGATTGTAGTTCA	45.0	50.0	55.8	58.4	1196-1204 bp	1.0	60.4
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	1180-1188 bp	1.0	59.4
psbM-trnD-GUC_p3	AGGAACTAGAATGAACAGTGCA	TGTCCCGGGATTGTAGTTCA	40.9	50.0	57.4	58.4	1203-1211 bp	1.0	57.2
psbM-trnD-GUC_p4	AGAATGAACAGTGCAGTAGC	GTCCCGGGATTGTAGTTCAA	45.0	50.0	55.8	57.2	1195-1203 bp	1.0	57.0
psbM-trnD-GUC_p5	AGAATGAACAGTGCAGTAGC	TTGTCCCGGGATTGTAGTTC	45.0	50.0	55.8	57.2	1197-1205 bp	1.0	57.0