Hotspot

trnQ-UUG-psbK

LSC · 8000-8359 · 360 bp

Back to Dobinea

Pi 0.0111

MarkerSeek score 52.30

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 360
variable_sites: 4
pi: 0.0111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	AGTACACTCTGGGACGGAAG	CGGGCAATTTGGCGAATAAA	55.0	45.0	58.5	57.7	521-522 bp	1.0	74.7
trnQ-UUG-psbK_p2	GAGTACACTCTGGGACGGAA	CGGGCAATTTGGCGAATAAA	55.0	45.0	58.5	57.7	522-523 bp	1.0	74.7
trnQ-UUG-psbK_p3	GGATTCGAACCTCCGATAGC	CGGGCAATTTGGCGAATAAA	55.0	45.0	57.9	57.7	502 bp	0.5	57.2
trnQ-UUG-psbK_p4	GATTCGAACCTCCGATAGCG	CGGGCAATTTGGCGAATAAA	55.0	45.0	58.3	57.7	501 bp	0.5	55.2
trnQ-UUG-psbK_p5	GGATTCGAACCTCCGATAGC	TCGGGCAATTTGGCGAATAA	55.0	45.0	57.9	58.5	503 bp	0.5	55.2