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psbA-trnK-UUU

LSC · 1738-1991 · 254 bp

Back to Dobinea

Pi 0.0315

MarkerSeek score 68.10

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 254
variable_sites: 8
pi: 0.0315
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TACAGAAGCGACCCCATAGG	AATGAAAAATGCAGGCACGG	55.0	45.0	58.6	57.9	421 bp	1.0	76.1
psbA-trnK-UUU_p2	TACAGAAGCGACCCCATAGG	CGGCTTGGGGAGAGATTTTT	55.0	50.0	58.6	58.2	404 bp	1.0	75.6
psbA-trnK-UUU_p3	ATCCAGTTACAGAAGCGACC	AATGAAAAATGCAGGCACGG	50.0	45.0	57.3	57.9	428 bp	1.0	75.4
psbA-trnK-UUU_p4	AGTTACAGAAGCGACCCCAT	AATGAAAAATGCAGGCACGG	50.0	45.0	58.7	57.9	424 bp	1.0	75.1
psbA-trnK-UUU_p5	ATCCAGTTACAGAAGCGACC	CGGCTTGGGGAGAGATTTTT	50.0	50.0	57.3	58.2	411 bp	1.0	74.9