Hotspot

rpoC1

LSC · 22164-24951 · 2788 bp

Back to Distylium

Pi 0.000848

MarkerSeek score 47.90

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2788
variable_sites: 10
pi: 0.000848
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	GAGAGGACCGAATTCACACC	TTGCTCGTTCGAGAACTACG	55.0	50.0	58.0	58.0	2955-2963 bp	1.0	80.1
rpoC1_p2	GGAGAGGACCGAATTCACAC	TTGCTCGTTCGAGAACTACG	55.0	50.0	58.0	58.0	2956-2964 bp	1.0	80.1
rpoC1_p3	CCTAGTTGAACCGGAGAGGA	TTGCTCGTTCGAGAACTACG	55.0	50.0	58.2	58.0	2968-2976 bp	1.0	79.0
rpoC1_p4	GGACCGAATTCACACCGTAT	TTGCTCGTTCGAGAACTACG	50.0	50.0	57.7	58.0	2951-2959 bp	1.0	77.8
rpoC1_p5	GAGAGGACCGAATTCACACC	GCTCGTTCGAGAACTACGAT	55.0	50.0	58.0	57.5	2953-2961 bp	1.0	76.8