Hotspot

ndhF

SSC · 114072-116309 · 2238 bp

Back to Distylium

Pi 0.000873

MarkerSeek score 51.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2238
variable_sites: 9
pi: 0.000873
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GGAAGAGATCCGAGTGAATGG	AGATCTGGTGAATCGGAAACA	52.4	42.9	57.9	57.0	2411-2417 bp	1.0	55.7
ndhF_p2	GGAAGAGATCCGAGTGAATGG	AAGATCTGGTGAATCGGAAACA	52.4	40.9	57.9	57.7	2412-2418 bp	1.0	53.2
ndhF_p3	GGAAGAGATCCGAGTGAATGG	AGATCTGGTGAATCGGAAACAA	52.4	40.9	57.9	57.7	2411-2417 bp	1.0	53.2
ndhF_p4	TCCGAGTGAATGGAAAGGAA	AGATCTGGTGAATCGGAAACA	45.0	42.9	56.5	57.0	2403-2409 bp	1.0	52.2
ndhF_p5	GATCCGAGTGAATGGAAAGGA	AGATCTGGTGAATCGGAAACA	47.6	42.9	57.5	57.0	2405-2411 bp	1.0	52.2