Hotspot

ndhA

SSC · 124189-126362 · 2174 bp

Back to Distylium

Pi 0.00142

MarkerSeek score 56.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2174
variable_sites: 12
pi: 0.00142
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAGGGAACA	CCCGCCGGGTTTTATCAATT	47.6	50.0	58.1	58.9	2380-2394 bp	1.0	65.2
ndhA_p2	ACCCAGTTACCATAGGGAACA	CCGCCGGGTTTTATCAATTT	47.6	45.0	58.1	57.0	2379-2393 bp	1.0	64.8
ndhA_p3	TGAACCCAGTTACCATAGGGA	CCCGCCGGGTTTTATCAATT	47.6	50.0	57.8	58.9	2383-2397 bp	1.0	64.2
ndhA_p4	ACCCAGTTACCATAGGGAACA	GCCGGGTTTTATCAATTTGCA	47.6	42.9	58.1	58.0	2377-2391 bp	1.0	64.1
ndhA_p5	TGAACCCAGTTACCATAGGGA	CCGCCGGGTTTTATCAATTT	47.6	45.0	57.8	57.0	2382-2396 bp	1.0	63.7