Hotspot

ndhF-rpl32

SSC · 116310-117371 · 1062 bp

Back to Distylium

Pi 0.00148

MarkerSeek score 51.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1062
variable_sites: 8
pi: 0.00148
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCGTCGGAAAAAGGAGAAGT	AAAAGCTTTTAACGCTGCCC	50.0	45.0	57.8	57.9	1249-1253 bp	1.0	78.3
ndhF-rpl32_p2	TTTGTTGCCGTCGGAAAAAG	AAAAGCTTTTAACGCTGCCC	45.0	45.0	57.8	57.9	1256-1260 bp	1.0	78.0
ndhF-rpl32_p3	CTGGAAGTGGAACGAAAGGT	AAAAGCTTTTAACGCTGCCC	50.0	45.0	57.7	57.9	1207-1211 bp	1.0	77.6
ndhF-rpl32_p4	CCGTCGGAAAAAGGAGAAGT	GCCCAATACCCCTTCCTTTT	50.0	50.0	57.8	57.8	1233-1237 bp	1.0	77.5
ndhF-rpl32_p5	TTTGTTGCCGTCGGAAAAAG	GCCCAATACCCCTTCCTTTT	45.0	50.0	57.8	57.8	1240-1244 bp	1.0	77.2