Hotspot

rpl20-rps12

LSC · 72500-73307 · 808 bp

Back to Distylium

Pi 0.000938

MarkerSeek score 54.20

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 808
variable_sites: 3
pi: 0.000938
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TTTGTTCTACGTCTCCGAGC	CGCCGAGGAACATGTACTAG	50.0	55.0	57.7	57.9	865-887 bp	1.0	79.2
rpl20-rps12_p2	TGTTCTACGTCTCCGAGCTA	CGCCGAGGAACATGTACTAG	50.0	55.0	57.6	57.9	863-885 bp	1.0	78.8
rpl20-rps12_p3	TTGTTCTACGTCTCCGAGCT	CGCCGAGGAACATGTACTAG	50.0	55.0	58.5	57.9	864-886 bp	1.0	78.2
rpl20-rps12_p4	CCCGAAAGCTTGATGCAAAT	CGCCGAGGAACATGTACTAG	45.0	55.0	57.3	57.9	894-916 bp	1.0	76.6
rpl20-rps12_p5	TTTGTTCTACGTCTCCGAGC	GCCGAGGAACATGTACTAGG	50.0	55.0	57.7	57.5	864-886 bp	1.0	76.1