Hotspot

rbcL-accD

LSC · 59953-60570 · 618 bp

Back to Distylium

Pi 0.00201

MarkerSeek score 54.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 618
variable_sites: 4
pi: 0.00201
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	ACATCGAATTGAACCACCGT	55.0	45.0	58.0	57.8	775-791 bp	1.0	80.2
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	TAGAGCTTTCTTGCCCCCTA	55.0	50.0	58.0	57.7	748-764 bp	1.0	79.6
rbcL-accD_p3	GAGGCTAGCAAATGGAGTCC	CATAGAGCTTTCTTGCCCCC	55.0	55.0	58.0	58.3	750-766 bp	1.0	79.2
rbcL-accD_p4	GAGGCTAGCAAATGGAGTCC	CCATAGAGCTTTCTTGCCCC	55.0	55.0	58.0	58.3	751-767 bp	1.0	79.2
rbcL-accD_p5	GAGGCTAGCAAATGGAGTCC	AGAGCTTTCTTGCCCCCTAT	55.0	50.0	58.0	58.4	747-763 bp	1.0	78.5