Hotspot

trnC-GCA-petN

LSC · 29426-30265 · 840 bp

Back to Distylium

Pi 0.00107
MarkerSeek score 59.00
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
840
variable_sites
5
pi
0.00107
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG ACGAGCGAAAGGGAAAATGT 50.0 45.0 58.1 58.1 935-950 bp 1.0 81.1
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG CCATACTACGAGCGAAAGGG 50.0 55.0 58.1 57.9 942-957 bp 1.0 80.9
trnC-GCA-petN_p3 TTTCCCCAGTTCAAATCCGG CCCATACTACGAGCGAAAGG 50.0 55.0 58.1 57.9 943-958 bp 1.0 80.9
trnC-GCA-petN_p4 TTTCCCCAGTTCAAATCCGG CCCCATACTACGAGCGAAAG 50.0 55.0 58.1 57.9 944-959 bp 1.0 80.9
trnC-GCA-petN_p5 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 897-912 bp 1.0 80.9