Hotspot

rpoB-trnC-GCA

LSC · 28191-29344 · 1154 bp

Back to Distylium

Pi 0.000985

MarkerSeek score 57.40

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1154
variable_sites: 6
pi: 0.000985
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGTCAAGCCCTGATCAATGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1305-1312 bp	1.0	80.8
rpoB-trnC-GCA_p2	CGTCAAGCCCTGATCAATGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.0	58.4	1287-1294 bp	1.0	78.6
rpoB-trnC-GCA_p3	AAAGTTCTTCCGTCAAGCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.4	58.1	1315-1322 bp	1.0	77.8
rpoB-trnC-GCA_p4	ATTCCCTCATTTCCATCCCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.3	58.1	1235-1242 bp	1.0	75.8
rpoB-trnC-GCA_p5	AAAGTTCTTCCGTCAAGCCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.4	58.4	1297-1304 bp	1.0	75.7