Hotspot

atpH-atpI

LSC · 14807-15940 · 1134 bp

Back to Distylium

Pi 0.000588

MarkerSeek score 51.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1134
variable_sites: 4
pi: 0.000588
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACAGAAGCGGCAGAAATCA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.1	57.8	1180-1193 bp	1.0	79.0
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	CGAATCCATGGAGGGTCATC	50.0	55.0	57.9	57.8	1238-1251 bp	1.0	78.9
atpH-atpI_p3	CAGCAGCAATAACAGAAGCG	CGAATCCATGGAGGGTCATC	50.0	55.0	57.8	57.8	1190-1203 bp	1.0	78.4
atpH-atpI_p4	GCAGTACCTTGACCAACTCC	CGAATCCATGGAGGGTCATC	55.0	55.0	58.2	57.8	1242-1255 bp	1.0	78.2
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	CGAATCCATGGAGGGTCATC	50.0	55.0	57.8	57.8	1197-1210 bp	1.0	78.1