Hotspot

trnS-GCU-trnG-GCC

LSC · 9259-10098 · 840 bp

Back to Distylium

Pi 0.00168

MarkerSeek score 43.90

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 840
variable_sites: 7
pi: 0.00168
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	GAATTAAACCGAGGGACCCC	50.0	55.0	58.0	58.0	952-965 bp	1.0	80.2
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	GAATTAAACCGAGGGACCCC	50.0	55.0	58.0	58.0	951-964 bp	1.0	80.2
trnS-GCU-trnG-GCC_p3	ACGGAAAGAGAGGGATTCGA	GAATTAAACCGAGGGACCCC	50.0	55.0	58.2	58.0	1006-1019 bp	1.0	79.0
trnS-GCU-trnG-GCC_p4	TGGAAACGGAAAGAGAGGGA	GAATTAAACCGAGGGACCCC	50.0	55.0	58.3	58.0	1011-1024 bp	1.0	78.0
trnS-GCU-trnG-GCC_p5	AGCAATCCGACGCTTTAGTC	GAATTAAACCGAGGGACCCC	50.0	55.0	58.4	58.0	954-967 bp	1.0	77.4