Hotspot

psbK-psbI

LSC · 8527-8864 · 338 bp

Back to Distylium

Pi 0.00345

MarkerSeek score 41.80

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 338
variable_sites: 7
pi: 0.00345
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CCAAATTACCCGAGGCCTAC	GGAATCCGAAGATGAAGAGAGA	55.0	45.5	58.0	57.1	569-570 bp	1.0	57.3
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	GGAATCCGAAGATGAAGAGAGA	50.0	45.5	57.9	57.1	483-484 bp	1.0	57.3
psbK-psbI_p3	CCAAATTACCCGAGGCCTAC	AGGAATCCGAAGATGAAGAGAG	55.0	45.5	58.0	57.1	570-571 bp	1.0	57.2
psbK-psbI_p4	GTTTGGCAAGCTGCTGTAAG	AGGAATCCGAAGATGAAGAGAG	50.0	45.5	57.9	57.1	484-485 bp	1.0	57.2
psbK-psbI_p5	CCAAATTACCCGAGGCCTAC	AGGAATCCGAAGATGAAGAGA	55.0	42.9	58.0	55.9	570-571 bp	1.0	55.7