Hotspot

rps18

LSC · 71551-71856 · 306 bp

Back to Distyliopsis

Pi 0.00654
MarkerSeek score 31.70
Primer available yes
Label rps18

Feature Metadata

label_name
rps18
feature_type
gene
region
LSC
length_bp
306
variable_sites
3
pi
0.00654
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
31.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps18_p1 TGTGATTGTGATTTGACCGA TGGAATTTTGATTCAATTGAAGAGT 40.0 28.0 55.3 55.9 388 bp 1.0 40.6
rps18_p2 TGTGATTGTGATTTGACCGA TTGATTCAATTGAAGAGTAAGC 40.0 31.8 55.3 52.8 381 bp 1.0 40.6
rps18_p3 TGTGATTGTGATTTGACCGAA TGGAATTTTGATTCAATTGAAGAGT 38.1 28.0 56.1 55.9 388 bp 1.0 40.6
rps18_p4 TTGTGATTGTGATTTGACCGA TGGAATTTTGATTCAATTGAAGAGT 38.1 28.0 56.1 55.9 389 bp 1.0 40.6
rps18_p5 TGTGATTGTGATTTGACCGA TTTGATTCAATTGAAGAGTAAGC 40.0 30.4 55.3 53.6 382 bp 1.0 40.6