Hotspot

rps16

LSC · 5132-6250 · 1119 bp

Back to Distyliopsis

Pi 0.00238

MarkerSeek score 57.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1119
variable_sites: 4
pi: 0.00238
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GTGATCCCCACACTCTTTTT	ACCGGTTCCAAACTAGAGGA	45.0	50.0	55.5	58.0	1180-1181 bp	1.0	60.9
rps16_p2	GTGATCCCCACACTCTTTTT	TGTACCGACTGAACCAATGAC	45.0	47.6	55.5	58.0	1233-1234 bp	1.0	52.8
rps16_p3	GTGATCCCCACACTCTTTTT	CCGGTTCCAAACTAGAGGAA	45.0	50.0	55.5	56.9	1179-1180 bp	1.0	52.0
rps16_p4	GTGATCCCCACACTCTTTTT	TACCGGTTCCAAACTAGAGGA	45.0	47.6	55.5	57.8	1181-1182 bp	1.0	51.7
rps16_p5	GTGATCCCCACACTCTTTTT	ACCGACTGAACCAATGACTAC	45.0	47.6	55.5	57.7	1230-1231 bp	1.0	50.6