Hotspot

ndhH

SSC · 126366-127547 · 1182 bp

Back to Distyliopsis

Pi 0.00226

MarkerSeek score 58.70

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 4
pi: 0.00226
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TCCCATAGACCTCTTTTACGGA	TCGTTTTCAGCAATTCATGGA	45.5	38.1	58.0	56.4	1302 bp	1.0	43.7
ndhH_p2	TCCCATAGACCTCTTTTACGGA	TCGTTTTCAGCAATTCATGGAA	45.5	36.4	58.0	57.2	1302 bp	1.0	41.4
ndhH_p3	TCCCATAGACCTCTTTTACGGA	TTCGTTTTCAGCAATTCATGGA	45.5	36.4	58.0	57.2	1303 bp	1.0	41.4
ndhH_p4	CCCATAGACCTCTTTTACGGA	TCGTTTTCAGCAATTCATGGA	47.6	38.1	56.5	56.4	1301 bp	1.0	40.2
ndhH_p5	TCCCATAGACCTCTTTTACGG	TCGTTTTCAGCAATTCATGGA	47.6	38.1	56.5	56.4	1302 bp	1.0	40.2