Hotspot

accD

LSC · 60571-62100 · 1530 bp

Back to Distyliopsis

Pi 0.00305

MarkerSeek score 46.50

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1530
variable_sites: 7
pi: 0.00305
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	GAGGCTAGCAAATGGAGTCC	GGCACCAAAACAGAGGGTAA	55.0	50.0	58.0	58.0	2966-2982 bp	1.0	80.2
accD_p2	ATATCCCGCAGTTTTGGACC	GGCACCAAAACAGAGGGTAA	50.0	50.0	57.9	58.0	2648-2664 bp	1.0	80.1
accD_p3	GAGGCTAGCAAATGGAGTCC	TCGTTTTAGTTGCCTTGCCT	55.0	45.0	58.0	58.0	2492-2495 bp	1.0	80.0
accD_p4	ATATCCCGCAGTTTTGGACC	TCGTTTTAGTTGCCTTGCCT	50.0	45.0	57.9	58.0	2174-2177 bp	1.0	80.0
accD_p5	TGATATCCCGGATTCCCACA	GGCACCAAAACAGAGGGTAA	50.0	50.0	57.9	58.0	2390-2406 bp	1.0	79.6