Hotspot

rpl32-trnL-UAG

SSC · 117536-118596 · 1061 bp

Back to Distyliopsis

Pi 0.00629

MarkerSeek score 51.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1061
variable_sites: 10
pi: 0.00629
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TTTGTGCCGACAAATACAAA	TCCTAAGAGCAGCGTGTCTA	35.0	50.0	54.3	57.9	1124-1127 bp	1.0	51.2
rpl32-trnL-UAG_p2	TTTGTGCCGACAAATACAAA	AGCAGCGTGTCTACCAATTT	35.0	45.0	54.3	57.8	1117-1120 bp	1.0	50.7
rpl32-trnL-UAG_p3	TTTGTGCCGACAAATACAAA	CCTAAGAGCAGCGTGTCTAC	35.0	55.0	54.3	57.8	1123-1126 bp	1.0	50.6
rpl32-trnL-UAG_p4	TTTGTGCCGACAAATACAAA	CTAAGAGCAGCGTGTCTACC	35.0	55.0	54.3	57.8	1122-1125 bp	1.0	50.6
rpl32-trnL-UAG_p5	TTTGTGCCGACAAATACAAA	GAGCAGCGTGTCTACCAATT	35.0	50.0	54.3	58.3	1118-1121 bp	1.0	50.0