Hotspot

petA-psbJ

LSC · 66649-67704 · 1056 bp

Back to Distyliopsis

Pi 0.0101

MarkerSeek score 53.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1056
variable_sites: 16
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	ACTGGTATTCCTGTGATCGG	50.0	50.0	57.8	56.7	1233-1242 bp	1.0	69.8
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	CTGGTATTCCTGTGATCGGT	50.0	50.0	57.8	56.7	1232-1241 bp	1.0	69.8
petA-psbJ_p3	GATCCATTACGGGTCCAAGG	ACTGGTATTCCTGTGATCGG	55.0	50.0	57.7	56.7	1251-1260 bp	1.0	69.0
petA-psbJ_p4	GATCCATTACGGGTCCAAGG	CTGGTATTCCTGTGATCGGT	55.0	50.0	57.7	56.7	1250-1259 bp	1.0	69.0
petA-psbJ_p5	GGTCCAAGGCCTTTTGTTCT	CTGGTATTCCTGTGATCGGT	50.0	50.0	58.3	56.7	1239-1248 bp	1.0	68.7