Hotspot

trnL-UAA-trnF-GAA

LSC · 50802-51175 · 374 bp

Back to Distyliopsis

Pi 0.00536

MarkerSeek score 60.20

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 374
variable_sites: 3
pi: 0.00536
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.5	58.0	426-429 bp	1.0	78.4
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	462-465 bp	1.0	77.9
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	GTGCCAGGAACCAGATTTGA	50.0	50.0	57.5	58.1	477-480 bp	1.0	77.5
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAG	50.0	55.0	57.5	57.9	438-441 bp	1.0	77.3
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	CATGTGCCAGGAACCAGATT	50.0	50.0	57.5	58.2	480-483 bp	1.0	76.9