Hotspot

trnE-UUC-trnT-GGU

LSC · 33500-34379 · 880 bp

Back to Distyliopsis

Pi 0.00152

MarkerSeek score 60.80

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 880
variable_sites: 2
pi: 0.00152
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	1025 bp	1.0	79.5
trnE-UUC-trnT-GGU_p2	TTTCGTAGTACCCTACCCCC	TACCGCTGAGTTAAAAGGGC	55.0	50.0	57.8	57.9	990 bp	1.0	78.7
trnE-UUC-trnT-GGU_p3	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	987 bp	1.0	78.7
trnE-UUC-trnT-GGU_p4	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	55.0	55.0	57.8	58.2	1022 bp	1.0	78.3
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.3	57.9	952 bp	1.0	77.9