Hotspot

trnC-GCA-petN

LSC · 29423-30262 · 840 bp

Back to Distyliopsis

Pi 0.00397

MarkerSeek score 60.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 840
variable_sites: 5
pi: 0.00397
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	ACGAGCGAAAGGGAAAATGT	50.0	45.0	58.1	58.1	944-949 bp	1.0	79.7
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CCATACTACGAGCGAAAGGG	50.0	55.0	58.1	57.9	951-956 bp	1.0	79.5
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CCCATACTACGAGCGAAAGG	50.0	55.0	58.1	57.9	952-957 bp	1.0	79.5
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	CCCCATACTACGAGCGAAAG	50.0	55.0	58.1	57.9	953-958 bp	1.0	79.5
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	906-911 bp	1.0	79.5