Hotspot

atpF-atpH

LSC · 14116-14566 · 451 bp

Back to Distyliopsis

Pi 0.00887

MarkerSeek score 61.60

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 451
variable_sites: 6
pi: 0.00887
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAGTGACCCAAGGAAACGAA	CGGAGGGAAAAATACGAGGT	50.0	50.0	58.0	57.3	604-605 bp	1.0	75.1
atpF-atpH_p2	CAGTGACCCAAGGAAACGAA	CGAGGCGGAGGGAAAAATAC	50.0	55.0	58.0	58.7	609-610 bp	1.0	75.0
atpF-atpH_p3	CAGTGACCCAAGGAAACGAA	GAGGCGGAGGGAAAAATACG	50.0	55.0	58.0	58.7	608-609 bp	1.0	75.0
atpF-atpH_p4	CAGTGACCCAAGGAAACGAA	GCGGAGGGAAAAATACGAGG	50.0	55.0	58.0	58.7	605-606 bp	1.0	75.0
atpF-atpH_p5	CAGTGACCCAAGGAAACGAA	GGCGGAGGGAAAAATACGAG	50.0	55.0	58.0	58.7	606-607 bp	1.0	75.0