Hotspot

atpA-atpF

LSC · 12758-12822 · 65 bp

Back to Distyliopsis

Pi 0.0104
MarkerSeek score 60.60
Primer available yes
Label atpA-atpF

Feature Metadata

label_name
atpA-atpF
feature_type
igs
region
LSC
length_bp
65
variable_sites
1
pi
0.0104
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA-atpF_p1 CACTTGAAGTACGGTACCGG TGTTTGGGGCGATGAAAGAA 55.0 45.0 58.0 58.0 200-202 bp 1.0 81.0
atpA-atpF_p2 CCACTTGAAGTACGGTACCG TGTTTGGGGCGATGAAAGAA 55.0 45.0 58.0 58.0 201-203 bp 1.0 81.0
atpA-atpF_p3 CACTTGAAGTACGGTACCGG ATGTTTGGGGCGATGAAAGA 55.0 45.0 58.0 57.8 201-203 bp 1.0 79.4
atpA-atpF_p4 CCACTTGAAGTACGGTACCG ATGTTTGGGGCGATGAAAGA 55.0 45.0 58.0 57.8 202-204 bp 1.0 79.4
atpA-atpF_p5 CACTTGAAGTACGGTACCGG GGTATGTTTGGGGCGATGAA 55.0 50.0 58.0 58.2 204-206 bp 1.0 79.1