Hotspot

trnK-UUU-rps16

LSC · 4280-5131 · 852 bp

Back to Distyliopsis

Pi 0.00861

MarkerSeek score 53.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 852
variable_sites: 11
pi: 0.00861
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	AGAAGGCGGAGGTTTTTACG	50.0	50.0	58.2	58.2	939-946 bp	1.0	78.6
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	AGAAGGCGGAGGTTTTTACG	50.0	50.0	58.2	58.2	938-945 bp	1.0	78.6
trnK-UUU-rps16_p3	CCGCACTTAAAAGCCGAGTA	AGAAGGCGGAGGTTTTTACG	50.0	50.0	58.3	58.2	948-955 bp	1.0	77.7
trnK-UUU-rps16_p4	AAAGCCGAGTACTCTACCGT	GGTTTTTACGGAACTTCGCC	50.0	50.0	58.2	57.7	929-936 bp	1.0	77.6
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	GGTTTTTACGGAACTTCGCC	50.0	50.0	58.2	57.7	928-935 bp	1.0	77.6