Hotspot

trnH-GUG-psbA

LSC · 83-445 · 363 bp

Back to Distyliopsis

Pi 0.0129

MarkerSeek score 35.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 363
variable_sites: 7
pi: 0.0129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TCAGTGCTATGCATGGTTCC	55.0	50.0	58.1	58.0	816-824 bp	1.0	79.5
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.9	58.0	830-838 bp	1.0	79.5
trnH-GUG-psbA_p3	GATCCACTTGGCTACATCCG	CAGTGCTATGCATGGTTCCT	55.0	50.0	58.1	58.0	815-823 bp	1.0	79.5
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.9	58.0	829-837 bp	1.0	79.4
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.9	58.0	831-839 bp	1.0	79.4