Hotspot

rpoC2

LSC · 25498-30027 · 4530 bp

Back to Distichlis

Pi 0.0151

MarkerSeek score 48.60

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4530
variable_sites: 68
pi: 0.0151
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CATCCTTTTCGCTGCACAAG	AACTCGGACCAATCCACCTA	50.0	50.0	58.0	58.1	1037-1049 bp	1.0	84.0
rpoC2_p2	CATCCTTTTCGCTGCACAAG	TGGTGGTATCAAACTAGCGC	50.0	50.0	58.0	58.0	1136-1148 bp	1.0	83.9
rpoC2_p3	ATAGCATCCTTTTCGCTGCA	TGGTGGTATCAAACTAGCGC	45.0	50.0	58.0	58.0	1140-1152 bp	1.0	83.8
rpoC2_p4	CATCCTTTTCGCTGCACAAG	TGCAGGTCGAACTGAAACAA	50.0	45.0	58.0	58.0	1250-1262 bp	1.0	83.4
rpoC2_p5	ATAGCATCCTTTTCGCTGCA	TGCAGGTCGAACTGAAACAA	45.0	45.0	58.0	58.0	1254-1266 bp	1.0	83.3