Hotspot

petG-trnW-CCA

LSC · 64312-64437 · 126 bp

Back to Distichlis

Pi 0.0806

MarkerSeek score 82.80

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 126
variable_sites: 10
pi: 0.0806
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	GGGATTATTCGTGACTGCGT	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.1	57.9	221-223 bp	1.0	83.1
petG-trnW-CCA_p2	GCGTATTTGCAATACAGGCG	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.3	57.9	205-207 bp	1.0	81.9
petG-trnW-CCA_p3	AGCGGGATTATTCGTGACTG	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.8	57.9	224-226 bp	1.0	81.8
petG-trnW-CCA_p4	GGGATTATTCGTGACTGCGT	CCCGATGTCGTAGGTTCAAA	50.0	50.0	58.1	57.6	216-218 bp	1.0	80.9
petG-trnW-CCA_p5	GGGATTATTCGTGACTGCGT	TCCAAAACCCGATGTCGTAG	50.0	50.0	58.1	57.6	223-225 bp	1.0	80.8