Hotspot

atpA-trnR-UCU

LSC · 36567-36747 · 181 bp

Back to Distichlis

Pi 0.0316

MarkerSeek score 65.50

Primer available yes

Label atpA-trnR-UCU

Feature Metadata

label_name: atpA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 181
variable_sites: 5
pi: 0.0316
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-trnR-UCU_p1	TGAACGGTTTTCCCTTCAGG	GGTTCAAATCCTATTGGACGC	50.0	47.6	58.0	57.3	219-234 bp	1.0	72.0
atpA-trnR-UCU_p2	TAGCAAGACATTCACCGAGC	GGTTCAAATCCTATTGGACGC	50.0	47.6	58.0	57.3	279-294 bp	1.0	71.8
atpA-trnR-UCU_p3	AGGAACAGCTTGAACGGTTT	GGTTCAAATCCTATTGGACGC	45.0	47.6	57.9	57.3	229-244 bp	1.0	71.7
atpA-trnR-UCU_p4	AGCTTGAACGGTTTTCCCTT	GGTTCAAATCCTATTGGACGC	45.0	47.6	57.9	57.3	223-238 bp	1.0	71.7
atpA-trnR-UCU_p5	GAACGGTTTTCCCTTCAGGA	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.7	57.3	218-233 bp	1.0	70.1