Hotspot

atpF-atpA

LSC · 34949-35042 · 94 bp

Back to Distichlis

Pi 0.0319

MarkerSeek score 67.00

Primer available yes

Label atpF-atpA

Feature Metadata

label_name: atpF-atpA
feature_type: igs
region: LSC
length_bp: 94
variable_sites: 3
pi: 0.0319
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpA_p1	CCGTACAAGGAGCTCTAGGA	CATCCCCCACTTGAACTACG	55.0	55.0	57.9	58.0	316 bp	1.0	80.1
atpF-atpA_p2	AAGCCGTACAAGGAGCTCTA	CATCCCCCACTTGAACTACG	50.0	55.0	58.2	58.0	319 bp	1.0	79.3
atpF-atpA_p3	CCGTACAAGGAGCTCTAGGA	TTGTTCAATACGTTCGCGGA	55.0	45.0	57.9	58.2	261 bp	1.0	78.7
atpF-atpA_p4	ATTTCCGTACGATTCGTGCT	CATCCCCCACTTGAACTACG	45.0	55.0	57.7	58.0	265 bp	1.0	78.4
atpF-atpA_p5	AAGCCGTACAAGGAGCTCTA	TTGTTCAATACGTTCGCGGA	50.0	45.0	58.2	58.2	264 bp	1.0	77.9