Hotspot

ndhD-psaC

SSC · 119095-119233 · 139 bp

Back to Dissochaeta

Pi 0.048

MarkerSeek score 83.10

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 139
variable_sites: 10
pi: 0.048
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AGAAAATTAAAGAACCCGCCA	ATGGCATGAAACCACTCGAA	38.1	45.0	56.0	57.8	246-249 bp	1.0	58.0
ndhD-psaC_p2	AGAAAATTAAAGAACCCGCCA	CACTCGAAGCATGGGTCTAG	38.1	55.0	56.0	57.8	234-237 bp	1.0	58.0
ndhD-psaC_p3	AGAAAATTAAAGAACCCGCCA	AGAGATGTGAATCCGCTTGC	38.1	50.0	56.0	58.3	298-301 bp	1.0	56.3
ndhD-psaC_p4	AAGAAAATTAAAGAACCCGCCA	ATGGCATGAAACCACTCGAA	36.4	45.0	56.8	57.8	247-250 bp	1.0	55.9
ndhD-psaC_p5	AGAAAATTAAAGAACCCGCCAA	ATGGCATGAAACCACTCGAA	36.4	45.0	56.8	57.8	246-249 bp	1.0	55.9