Hotspot

psbE-petL

LSC · 66048-67196 · 1149 bp

Back to Dissochaeta

Pi 0.0138

MarkerSeek score 63.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1149
variable_sites: 20
pi: 0.0138
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	TGGGTTGTGCTCATTCAATTG	50.0	42.9	57.8	57.3	1132-1296 bp	1.0	70.8
psbE-petL_p2	ACGAATAACCAACCCGCAAT	TGGGTTGTGCTCATTCAATTG	45.0	42.9	58.2	57.3	1218-1382 bp	1.0	70.6
psbE-petL_p3	TGCTGACGAATAACCAACCC	TGGGTTGTGCTCATTCAATTG	50.0	42.9	58.2	57.3	1223-1387 bp	1.0	70.5
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	TGGGTTGTGCTCATTCAATTG	55.0	42.9	58.2	57.3	1127-1291 bp	1.0	70.5
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	TGGGTTGTGCTCATTCAATTG	50.0	42.9	58.4	57.3	1125-1289 bp	1.0	69.2