Hotspot

rbcL-accD

LSC · 58071-58731 · 661 bp

Back to Dissochaeta

Pi 0.0154

MarkerSeek score 69.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 661
variable_sites: 15
pi: 0.0154
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTGCTTGTGAGGTATGGAA	TTTCCCGCTAGACAACATCG	50.0	50.0	57.9	58.0	753-763 bp	1.0	81.7
rbcL-accD_p2	GCTGCTTGTGAGGTATGGAA	GAATTAAACCGCCGTCGTTC	50.0	50.0	57.9	57.9	734-744 bp	1.0	80.6
rbcL-accD_p3	GAGTCCTGAACTAGCTGCTG	TTTCCCGCTAGACAACATCG	55.0	50.0	57.7	58.0	769-779 bp	1.0	80.2
rbcL-accD_p4	GCTGCTTGTGAGGTATGGAA	CCCGCTAGACAACATCGAAT	50.0	50.0	57.9	57.8	750-760 bp	1.0	79.9
rbcL-accD_p5	GCTGCTTGTGAGGTATGGAA	ACATCGAATTAAACCGCCGT	50.0	45.0	57.9	58.3	739-749 bp	1.0	79.6