Hotspot

trnF-GAA-ndhJ

LSC · 49681-50529 · 849 bp

Back to Dissochaeta

Pi 0.0145

MarkerSeek score 69.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 849
variable_sites: 17
pi: 0.0145
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ACTCATCCACGACTGAAACG	55.0	50.0	58.0	57.9	1013-1030 bp	1.0	82.0
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	CTCATCCACGACTGAAACGT	55.0	50.0	58.0	57.9	1012-1029 bp	1.0	82.0
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	977-994 bp	1.0	81.9
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	CTCATCCACGACTGAAACGT	50.0	50.0	58.1	57.9	976-993 bp	1.0	81.7
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ACTCATCCACGACTGAAACG	50.0	50.0	58.1	57.9	977-994 bp	1.0	81.6