Hotspot

psbZ-trnG-UCC

LSC · 37433-37960 · 528 bp

Back to Dissochaeta

Pi 0.0197

MarkerSeek score 73.10

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 528
variable_sites: 15
pi: 0.0197
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	GACGGCTGGTCAAGTAACAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.1	57.8	663-674 bp	1.0	81.8
psbZ-trnG-UCC_p2	CCCGTTGTTTTTGCTTCTCC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.9	57.8	684-695 bp	1.0	81.6
psbZ-trnG-UCC_p3	ACGGCTGGTCAAGTAACAAA	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.7	57.8	662-673 bp	1.0	80.2
psbZ-trnG-UCC_p4	GTTTTTGCTTCTCCTGACGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.3	57.8	678-689 bp	1.0	77.2
psbZ-trnG-UCC_p5	TTTTTGCTTCTCCTGACGGC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	59.0	57.8	677-688 bp	1.0	74.4