Hotspot

trnD-GUC-trnY-GUA

LSC · 31148-31611 · 464 bp

Back to Dissochaeta

Pi 0.0141

MarkerSeek score 63.40

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 464
variable_sites: 9
pi: 0.0141
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	GCGGTGCTCTAACCAATTGA	ATGGATTCTTCCTGGGTCGA	50.0	50.0	58.6	58.1	576-592 bp	1.0	78.1
trnD-GUC-trnY-GUA_p2	GCGGTGCTCTAACCAATTGA	TGGATTCTTCCTGGGTCGAT	50.0	50.0	58.6	58.1	575-591 bp	1.0	78.1
trnD-GUC-trnY-GUA_p3	GCGGTGCTCTAACCAATTGA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.6	57.8	519-535 bp	1.0	77.5
trnD-GUC-trnY-GUA_p4	TTTATTCCGTCGGGACTGAC	ATGGATTCTTCCTGGGTCGA	50.0	50.0	57.3	58.1	629-645 bp	1.0	76.9
trnD-GUC-trnY-GUA_p5	TTTATTCCGTCGGGACTGAC	TGGATTCTTCCTGGGTCGAT	50.0	50.0	57.3	58.1	628-644 bp	1.0	76.9