Hotspot

trnC-GCA-petN

LSC · 28937-29771 · 835 bp

Back to Dissochaeta

Pi 0.0184

MarkerSeek score 66.00

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 835
variable_sites: 23
pi: 0.0184
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	926-952 bp	1.0	79.1
trnC-GCA-petN_p2	ATTTTGGTTTGGCGACATGG	TTAAAGCAGCCCAAGCAAGA	45.0	45.0	57.5	58.0	960-986 bp	1.0	78.2
trnC-GCA-petN_p3	AGTTCAAATCTGGGTGTCGC	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.5	58.0	901-927 bp	1.0	78.1
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.4	57.8	920-946 bp	1.0	77.6
trnC-GCA-petN_p5	ATTTTGGTTTGGCGACATGG	CAGCCCAAGCAAGACTTACT	45.0	50.0	57.5	57.8	954-980 bp	1.0	76.7