Hotspot

rpoB-trnC-GCA

LSC · 27416-28855 · 1440 bp

Back to Dissochaeta

Pi 0.0142

MarkerSeek score 62.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1440
variable_sites: 30
pi: 0.0142
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCACTTCCATCCACCAGCAT	AAAAGGATTTGCAGTCCCCC	50.0	50.0	59.0	58.4	1504-1507 bp	1.0	72.2
rpoB-trnC-GCA_p2	TTCCCTCACTTCCATCCACC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	59.0	58.4	1509-1512 bp	1.0	72.2
rpoB-trnC-GCA_p3	GACATTCCCTCACTTCCATCC	AAAAGGATTTGCAGTCCCCC	52.4	50.0	58.1	58.4	1513-1516 bp	1.0	71.7
rpoB-trnC-GCA_p4	CATTCCCTCACTTCCATCCA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	56.9	58.4	1511-1514 bp	1.0	71.4
rpoB-trnC-GCA_p5	ATTCCCTCACTTCCATCCAC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	56.9	58.4	1510-1513 bp	1.0	71.4