Hotspot

atpF-atpH

LSC · 13340-13950 · 611 bp

Back to Dissochaeta

Pi 0.023

MarkerSeek score 46.90

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 611
variable_sites: 21
pi: 0.023
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GGCCCAAGGAAAGGAAAGAA	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	58.0	58.0	882-899 bp	1.0	81.8
atpF-atpH_p2	GGCCCAAGGAAAGGAAAGAA	TTAGCTGTCGGACTTGCTTC	50.0	50.0	58.0	57.9	852-869 bp	1.0	81.5
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	57.9	58.0	914-931 bp	1.0	80.8
atpF-atpH_p4	GGCCCAAGGAAAGGAAAGAA	TTTCTGCCGCTTCCGTTATT	50.0	45.0	58.0	58.2	881-898 bp	1.0	80.6
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	TTAGCTGTCGGACTTGCTTC	50.0	50.0	57.9	57.9	884-901 bp	1.0	80.6