primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score rps16-trnQ-UUG_p1 rps16-trnQ-UUG 1 AAGAATCCACGAACGATCCC GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 57.7 58.1 0.1 16.9 0.387225 5716 6878 1263-1273 1.000000 80.4 rps16-trnQ-UUG_p2 rps16-trnQ-UUG 2 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 57.2 58.1 0.0 16.9 0.863824 5716 6878 1228-1238 1.000000 76.7 rps16-trnQ-UUG_p3 rps16-trnQ-UUG 3 AAGAATCCACGAACGATCCC TTCGGAGGTTCGAATCCTTC 20 20 50.0 50.0 57.7 57.3 0.1 16.9 0.978597 5716 6878 1267-1277 1.000000 75.7 rps16-trnQ-UUG_p4 rps16-trnQ-UUG 4 AAGAATCCACGAACGATCCC TAAGGCAACGGGTTTTGGTC 20 20 50.0 50.0 57.7 58.7 0.1 0.0 0.990617 5716 6878 1293-1303 1.000000 75.5 rps16-trnQ-UUG_p5 rps16-trnQ-UUG 5 GCACGTTGCTTTCTACCACA GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 59.1 58.1 0.0 16.9 1.134559 5716 6878 1231-1241 1.000000 74.5 trnS-GCU-trnR-UCU_p1 trnS-GCU-trnR-UCU 1 ACGGAAAGAGAGGGATTCGA AGGTTTAGAAGACCTATGTCCT 20 22 50.0 40.9 58.2 55.6 0.0 0.0 4.525765 8088 9930 1970-1974 1.000000 46.1 trnS-GCU-trnR-UCU_p2 trnS-GCU-trnR-UCU 2 ACGGAAAGAGAGGGATTCGA AGAAGACCTATGTCCTATCCA 20 21 50.0 42.9 58.2 54.6 0.0 0.0 4.555847 8088 9930 1964-1968 1.000000 45.9 trnS-GCU-trnR-UCU_p3 trnS-GCU-trnR-UCU 3 TAGCAATCCGACGCTTTAGG AGGTTTAGAAGACCTATGTCCT 20 22 50.0 40.9 57.8 55.6 0.0 0.0 4.593370 8088 9930 1919-1923 1.000000 45.7 trnS-GCU-trnR-UCU_p4 trnS-GCU-trnR-UCU 4 ACGGAAAGAGAGGGATTCGA ACCAAAGGTTTAGAAGACCTATGT 20 24 50.0 37.5 58.2 57.6 0.0 0.0 4.601178 8088 9930 1975-1979 1.000000 45.5 trnS-GCU-trnR-UCU_p5 trnS-GCU-trnR-UCU 5 TAGCAATCCGACGCTTTAGG AGAAGACCTATGTCCTATCCA 20 21 50.0 42.9 57.8 54.6 0.0 0.0 4.623452 8088 9930 1913-1917 1.000000 45.4 atpF-atpH_p1 atpF-atpH 1 GGCCCAAGGAAAGGAAAGAA ATTTCTGCCGCTTCCGTTAT 20 20 50.0 45.0 58.0 58.0 0.0 0.0 0.048068 13340 13950 882-899 1.000000 81.8 atpF-atpH_p2 atpF-atpH 2 GGCCCAAGGAAAGGAAAGAA TTAGCTGTCGGACTTGCTTC 20 20 50.0 50.0 58.0 57.9 0.0 25.2 0.089967 13340 13950 852-869 1.000000 81.5 atpF-atpH_p3 atpF-atpH 3 ATTAAACCCGAAACTCCCGG ATTTCTGCCGCTTCCGTTAT 20 20 50.0 45.0 57.9 58.0 0.0 0.0 0.156700 13340 13950 914-931 1.000000 80.8 atpF-atpH_p4 atpF-atpH 4 GGCCCAAGGAAAGGAAAGAA TTTCTGCCGCTTCCGTTATT 20 20 50.0 45.0 58.0 58.2 0.0 0.0 0.193607 13340 13950 881-898 1.000000 80.6 atpF-atpH_p5 atpF-atpH 5 ATTAAACCCGAAACTCCCGG TTAGCTGTCGGACTTGCTTC 20 20 50.0 50.0 57.9 57.9 0.0 25.2 0.198599 13340 13950 884-901 1.000000 80.6 atpH-atpI_p1 atpH-atpI 1 ATAACGGAAGCGGCAGAAAT TAGCCGCCGCTTATATAGGA 20 20 45.0 50.0 58.0 57.9 1.4 4.5 0.171668 14197 15144 1007-1024 1.000000 81.8 atpH-atpI_p2 atpH-atpI 2 ATAACGGAAGCGGCAGAAAT TTTTTGCAACTTTAGCCGCC 20 20 45.0 45.0 58.0 58.1 1.4 0.0 0.179004 14197 15144 1019-1036 1.000000 81.7 atpH-atpI_p3 atpH-atpI 3 GAAGCAAGTCCGACAGCTAA TAGCCGCCGCTTATATAGGA 20 20 50.0 50.0 57.9 57.9 16.1 4.5 0.213567 14197 15144 1037-1054 1.000000 81.4 atpH-atpI_p4 atpH-atpI 4 GAAGCAAGTCCGACAGCTAA TTTTTGCAACTTTAGCCGCC 20 20 50.0 45.0 57.9 58.1 16.1 0.0 0.220903 14197 15144 1049-1066 1.000000 81.3 atpH-atpI_p5 atpH-atpI 5 AATAACGGAAGCGGCAGAAA TAGCCGCCGCTTATATAGGA 20 20 45.0 50.0 58.2 57.9 1.4 4.5 0.317207 14197 15144 1008-1025 1.000000 80.6 rpoB-trnC-GCA_p1 rpoB-trnC-GCA 1 TCACTTCCATCCACCAGCAT AAAAGGATTTGCAGTCCCCC 20 20 50.0 50.0 59.0 58.4 0.0 14.6 1.361244 27416 28855 1504-1507 1.000000 72.2 rpoB-trnC-GCA_p2 rpoB-trnC-GCA 2 TTCCCTCACTTCCATCCACC AAAAGGATTTGCAGTCCCCC 20 20 55.0 50.0 59.0 58.4 0.0 14.6 1.363571 27416 28855 1509-1512 1.000000 72.2 rpoB-trnC-GCA_p3 rpoB-trnC-GCA 3 GACATTCCCTCACTTCCATCC AAAAGGATTTGCAGTCCCCC 21 20 52.4 50.0 58.1 58.4 0.0 14.6 1.431069 27416 28855 1513-1516 1.000000 71.7 rpoB-trnC-GCA_p4 rpoB-trnC-GCA 4 CATTCCCTCACTTCCATCCA AAAAGGATTTGCAGTCCCCC 20 20 50.0 50.0 56.9 58.4 0.0 14.6 1.463812 27416 28855 1511-1514 1.000000 71.4 rpoB-trnC-GCA_p5 rpoB-trnC-GCA 5 ATTCCCTCACTTCCATCCAC AAAAGGATTTGCAGTCCCCC 20 20 50.0 50.0 56.9 58.4 0.0 14.6 1.465977 27416 28855 1510-1513 1.000000 71.4 trnC-GCA-petN_p1 trnC-GCA-petN 1 GGGGGACTGCAAATCCTTTT TTAAAGCAGCCCAAGCAAGA 20 20 50.0 45.0 58.4 58.0 14.7 0.0 0.361535 28937 29771 926-952 1.000000 79.1 trnC-GCA-petN_p2 trnC-GCA-petN 2 ATTTTGGTTTGGCGACATGG TTAAAGCAGCCCAAGCAAGA 20 20 45.0 45.0 57.5 58.0 0.0 0.0 0.460027 28937 29771 960-986 1.000000 78.2 trnC-GCA-petN_p3 trnC-GCA-petN 3 AGTTCAAATCTGGGTGTCGC TTAAAGCAGCCCAAGCAAGA 20 20 50.0 45.0 58.5 58.0 0.0 0.0 0.482286 28937 29771 901-927 1.000000 78.1 trnC-GCA-petN_p4 trnC-GCA-petN 4 GGGGGACTGCAAATCCTTTT CAGCCCAAGCAAGACTTACT 20 20 50.0 50.0 58.4 57.8 14.7 0.0 0.546619 28937 29771 920-946 1.000000 77.6 trnC-GCA-petN_p5 trnC-GCA-petN 5 ATTTTGGTTTGGCGACATGG CAGCCCAAGCAAGACTTACT 20 20 45.0 50.0 57.5 57.8 0.0 0.0 0.645111 28937 29771 954-980 1.000000 76.7 trnD-GUC-trnY-GUA_p1 trnD-GUC-trnY-GUA 1 GCGGTGCTCTAACCAATTGA ATGGATTCTTCCTGGGTCGA 20 20 50.0 50.0 58.6 58.1 7.1 0.0 0.671441 31148 31611 576-592 1.000000 78.1 trnD-GUC-trnY-GUA_p2 trnD-GUC-trnY-GUA 2 GCGGTGCTCTAACCAATTGA TGGATTCTTCCTGGGTCGAT 20 20 50.0 50.0 58.6 58.1 7.1 0.0 0.671441 31148 31611 575-591 1.000000 78.1 trnD-GUC-trnY-GUA_p3 trnD-GUC-trnY-GUA 3 GCGGTGCTCTAACCAATTGA TGGCAATATGTCTACGCTGG 20 20 50.0 50.0 58.6 57.8 7.1 0.0 0.789957 31148 31611 519-535 1.000000 77.5 trnD-GUC-trnY-GUA_p4 trnD-GUC-trnY-GUA 4 TTTATTCCGTCGGGACTGAC ATGGATTCTTCCTGGGTCGA 20 20 50.0 50.0 57.3 58.1 12.3 0.0 0.780614 31148 31611 629-645 1.000000 76.9 trnD-GUC-trnY-GUA_p5 trnD-GUC-trnY-GUA 5 TTTATTCCGTCGGGACTGAC TGGATTCTTCCTGGGTCGAT 20 20 50.0 50.0 57.3 58.1 12.3 0.0 0.780614 31148 31611 628-644 1.000000 76.9 psbZ-trnG-UCC_p1 psbZ-trnG-UCC 1 GACGGCTGGTCAAGTAACAA CGTCTTCTCCTTGGCAAAGA 20 20 50.0 50.0 58.1 57.8 0.0 1.1 0.296135 37433 37960 663-674 1.000000 81.8 psbZ-trnG-UCC_p2 psbZ-trnG-UCC 2 CCCGTTGTTTTTGCTTCTCC CGTCTTCTCCTTGGCAAAGA 20 20 50.0 50.0 57.9 57.8 0.0 1.1 0.304034 37433 37960 684-695 1.000000 81.6 psbZ-trnG-UCC_p3 psbZ-trnG-UCC 3 ACGGCTGGTCAAGTAACAAA CGTCTTCTCCTTGGCAAAGA 20 20 45.0 50.0 57.7 57.8 0.0 1.1 0.505148 37433 37960 662-673 1.000000 80.2 psbZ-trnG-UCC_p4 psbZ-trnG-UCC 4 GTTTTTGCTTCTCCTGACGG CGTCTTCTCCTTGGCAAAGA 20 20 50.0 50.0 57.3 57.8 0.0 1.1 0.858197 37433 37960 678-689 1.000000 77.2 psbZ-trnG-UCC_p5 psbZ-trnG-UCC 5 TTTTTGCTTCTCCTGACGGC CGTCTTCTCCTTGGCAAAGA 20 20 50.0 50.0 59.0 57.8 0.0 1.1 1.218275 37433 37960 677-688 1.000000 74.4 trnF-GAA-ndhJ_p1 trnF-GAA-ndhJ 1 GGGATAGCTCAGCTGGTAGA ACTCATCCACGACTGAAACG 20 20 55.0 50.0 58.0 57.9 10.3 0.0 0.068498 49681 50529 1013-1030 1.000000 82.0 trnF-GAA-ndhJ_p2 trnF-GAA-ndhJ 2 GGGATAGCTCAGCTGGTAGA CTCATCCACGACTGAAACGT 20 20 55.0 50.0 58.0 57.9 10.3 0.0 0.068498 49681 50529 1012-1029 1.000000 82.0 trnF-GAA-ndhJ_p3 trnF-GAA-ndhJ 3 GGGATAGCTCAGCTGGTAGA AGTTGGATAGGATGGCCCTT 20 20 55.0 50.0 58.0 58.1 10.3 0.0 0.089643 49681 50529 977-994 1.000000 81.9 trnF-GAA-ndhJ_p4 trnF-GAA-ndhJ 4 CCTCGTGTCACCAGTTCAAA CTCATCCACGACTGAAACGT 20 20 50.0 50.0 58.1 57.9 0.0 0.0 0.117888 49681 50529 976-993 1.000000 81.7 trnF-GAA-ndhJ_p5 trnF-GAA-ndhJ 5 CCTCGTGTCACCAGTTCAAA ACTCATCCACGACTGAAACG 20 20 50.0 50.0 58.1 57.9 0.0 0.0 0.117888 49681 50529 977-994 1.000000 81.6 rbcL-accD_p1 rbcL-accD 1 GCTGCTTGTGAGGTATGGAA TTTCCCGCTAGACAACATCG 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.118443 58071 58731 753-763 1.000000 81.7 rbcL-accD_p2 rbcL-accD 2 GCTGCTTGTGAGGTATGGAA GAATTAAACCGCCGTCGTTC 20 20 50.0 50.0 57.9 57.9 0.0 0.0 0.263218 58071 58731 734-744 1.000000 80.6 rbcL-accD_p3 rbcL-accD 3 GAGTCCTGAACTAGCTGCTG TTTCCCGCTAGACAACATCG 20 20 55.0 50.0 57.7 58.0 13.4 0.0 0.296685 58071 58731 769-779 1.000000 80.2 rbcL-accD_p4 rbcL-accD 4 GCTGCTTGTGAGGTATGGAA CCCGCTAGACAACATCGAAT 20 20 50.0 50.0 57.9 57.8 0.0 0.0 0.338929 58071 58731 750-760 1.000000 79.9 rbcL-accD_p5 rbcL-accD 5 GCTGCTTGTGAGGTATGGAA ACATCGAATTAAACCGCCGT 20 20 50.0 45.0 57.9 58.3 0.0 0.0 0.384577 58071 58731 739-749 1.000000 79.6 petA-psbJ_p1 petA-psbJ 1 ACGTGTCCAAGGTCTTTTGT ATACTCAGGGTTGGGCTCAT 20 20 45.0 50.0 57.6 58.1 0.0 0.0 0.514447 64372 65233 1002-1024 1.000000 79.0 petA-psbJ_p2 petA-psbJ 2 ACGTGTCCAAGGTCTTTTGT GTTCATACTCAGGGTTGGGC 20 20 45.0 55.0 57.6 58.2 0.0 0.0 0.661872 64372 65233 1006-1028 1.000000 77.8 petA-psbJ_p3 petA-psbJ 3 ACGTGTCCAAGGTCTTTTGT TTCATACTCAGGGTTGGGCT 20 20 45.0 50.0 57.6 58.3 0.0 0.0 0.744484 64372 65233 1005-1027 1.000000 77.1 petA-psbJ_p4 petA-psbJ 4 ACGTGTCCAAGGTCTTTTGT GGTTCATACTCAGGGTTGGG 20 20 45.0 55.0 57.6 57.6 0.0 0.0 0.833368 64372 65233 1007-1029 1.000000 76.4 petA-psbJ_p5 petA-psbJ 5 TCCATTACGTGTCCAAGGTC ATACTCAGGGTTGGGCTCAT 20 20 50.0 50.0 57.2 58.1 0.0 0.0 0.856084 64372 65233 1008-1030 1.000000 76.2 psbE-petL_p1 psbE-petL 1 AGAACGTTCTCCTGTGCTTC TGGGTTGTGCTCATTCAATTG 20 21 50.0 42.9 57.8 57.3 24.9 0.0 1.820338 66048 67196 1132-1296 1.000000 70.8 psbE-petL_p2 psbE-petL 2 ACGAATAACCAACCCGCAAT TGGGTTGTGCTCATTCAATTG 20 21 45.0 42.9 58.2 57.3 0.0 0.0 1.835015 66048 67196 1218-1382 1.000000 70.6 psbE-petL_p3 psbE-petL 3 TGCTGACGAATAACCAACCC TGGGTTGTGCTCATTCAATTG 20 21 50.0 42.9 58.2 57.3 0.0 0.0 1.847920 66048 67196 1223-1387 1.000000 70.5 psbE-petL_p4 psbE-petL 4 GTTCTCCTGTGCTTCCAGAC TGGGTTGTGCTCATTCAATTG 20 21 55.0 42.9 58.2 57.3 0.9 0.0 1.861926 66048 67196 1127-1291 1.000000 70.5 psbE-petL_p5 psbE-petL 5 TCTCCTGTGCTTCCAGACAT TGGGTTGTGCTCATTCAATTG 20 21 50.0 42.9 58.4 57.3 0.9 0.0 2.014673 66048 67196 1125-1289 1.000000 69.2 petG-trnW-CCA_p1 petG-trnW-CCA 1 ACGTGGTGATCAGTTAGACC CAAAACCCGATGTCGTAGGT 20 20 50.0 50.0 57.0 57.9 0.0 1.4 1.121164 67601 67737 201-201 1.000000 76.6 petG-trnW-CCA_p2 petG-trnW-CCA 2 ACGTGGTGATCAGTTAGACC AGCGCTCTTAGTTCAGTTCG 20 20 50.0 50.0 57.0 58.0 0.0 2.6 1.048242 67601 67737 237-237 1.000000 76.3 petG-trnW-CCA_p3 petG-trnW-CCA 3 GACGTGGTGATCAGTTAGACC CAAAACCCGATGTCGTAGGT 21 20 52.4 50.0 58.1 57.9 0.0 1.4 1.211615 67601 67737 202-202 1.000000 75.9 petG-trnW-CCA_p4 petG-trnW-CCA 4 GACGTGGTGATCAGTTAGACC AGCGCTCTTAGTTCAGTTCG 21 20 52.4 50.0 58.1 58.0 0.0 2.6 1.138693 67601 67737 238-238 1.000000 75.6 petG-trnW-CCA_p5 petG-trnW-CCA 5 CGTGGTGATCAGTTAGACCT AGCGCTCTTAGTTCAGTTCG 20 20 50.0 50.0 56.7 58.0 0.0 2.6 1.344212 67601 67737 236-236 1.000000 74.0 trnW-CCA-trnP-UGG_p1 trnW-CCA-trnP-UGG 1 GCGTTCTACCGAACTGAACT AGACCGCTTGAAAGAAAGGG 20 20 50.0 50.0 57.9 58.1 0.0 0.0 0.164180 67812 68013 320-320 1.000000 79.7 trnW-CCA-trnP-UGG_p2 trnW-CCA-trnP-UGG 2 GCGTTCTACCGAACTGAACT CTTGGTAGCGCGTTTGTTTT 20 20 50.0 45.0 57.9 57.9 0.0 8.4 0.175650 67812 68013 289-289 1.000000 79.7 trnW-CCA-trnP-UGG_p3 trnW-CCA-trnP-UGG 3 ACCTACGACATCGGGTTTTG AGACCGCTTGAAAGAAAGGG 20 20 50.0 50.0 57.9 58.1 6.2 0.0 0.191390 67812 68013 347-347 1.000000 79.4 trnW-CCA-trnP-UGG_p4 trnW-CCA-trnP-UGG 4 ACCTACGACATCGGGTTTTG CTTGGTAGCGCGTTTGTTTT 20 20 50.0 45.0 57.9 57.9 6.2 8.4 0.202860 67812 68013 316-316 1.000000 79.4 trnW-CCA-trnP-UGG_p5 trnW-CCA-trnP-UGG 5 GCGTTCTACCGAACTGAACT TTGGTAGCGCGTTTGTTTTG 20 20 50.0 45.0 57.9 58.2 0.0 8.4 0.232908 67812 68013 288-288 1.000000 79.2 rps12-clpP1_p1 rps12-clpP1 1 TGACATTTCGGATTGGCTGT GTACAAAGAACGGGCAAACC 20 20 45.0 50.0 57.8 57.6 0.0 0.0 0.609612 71571 71751 348-353 1.000000 81.4 rps12-clpP1_p2 rps12-clpP1 2 TTTCGGATTGGCTGTCTTGT GTACAAAGAACGGGCAAACC 20 20 45.0 50.0 57.7 57.6 0.0 0.0 0.681138 71571 71751 343-348 1.000000 80.9 rps12-clpP1_p3 rps12-clpP1 3 TGACATTTCGGATTGGCTGT TTATGTCAGCAACGGAAGCC 20 20 45.0 50.0 57.8 58.6 0.0 0.0 0.749842 71571 71751 290-295 1.000000 80.7 rps12-clpP1_p4 rps12-clpP1 4 TTTCGGATTGGCTGTCTTGT TTATGTCAGCAACGGAAGCC 20 20 45.0 50.0 57.7 58.6 0.0 0.0 0.821367 71571 71751 285-290 1.000000 80.2 rps12-clpP1_p5 rps12-clpP1 5 TTCGGATTGGCTGTCTTGTG GTACAAAGAACGGGCAAACC 20 20 50.0 50.0 58.5 57.6 0.0 0.0 0.888117 71571 71751 342-347 1.000000 79.2 ndhF-rpl32_p1 ndhF-rpl32 1 TGTTGCCATTGGAAAAACGA TGAAAAAGCCTTCAACGCTG 20 20 40.0 45.0 56.7 57.5 6.9 0.0 1.747675 114893 115743 1049-1065 1.000000 70.7 ndhF-rpl32_p2 ndhF-rpl32 2 TGTTGCCATTGGAAAAACGA GAAAAAGCCTTCAACGCTGT 20 20 40.0 45.0 56.7 57.5 6.9 0.0 1.749367 114893 115743 1048-1064 1.000000 70.7 ndhF-rpl32_p3 ndhF-rpl32 3 TTGTTGCCATTGGAAAAACGA TGAAAAAGCCTTCAACGCTG 21 20 38.1 45.0 57.5 57.5 6.9 0.0 2.017259 114893 115743 1050-1066 1.000000 68.5 ndhF-rpl32_p4 ndhF-rpl32 4 TTGTTGCCATTGGAAAAACGA GAAAAAGCCTTCAACGCTGT 21 20 38.1 45.0 57.5 57.5 6.9 0.0 2.018951 114893 115743 1049-1065 1.000000 68.5 ndhF-rpl32_p5 ndhF-rpl32 5 TGTTGCCATTGGAAAAACGAT TGAAAAAGCCTTCAACGCTG 21 20 38.1 45.0 57.3 57.5 6.9 0.0 2.232761 114893 115743 1049-1065 1.000000 66.8 ndhD-psaC_p1 ndhD-psaC 1 AGAAAATTAAAGAACCCGCCA ATGGCATGAAACCACTCGAA 21 20 38.1 45.0 56.0 57.8 0.0 0.0 3.152384 119095 119233 246-249 1.000000 58.0 ndhD-psaC_p2 ndhD-psaC 2 AGAAAATTAAAGAACCCGCCA CACTCGAAGCATGGGTCTAG 21 20 38.1 55.0 56.0 57.8 0.0 0.0 3.169464 119095 119233 234-237 1.000000 58.0 ndhD-psaC_p3 ndhD-psaC 3 AGAAAATTAAAGAACCCGCCA AGAGATGTGAATCCGCTTGC 21 20 38.1 50.0 56.0 58.3 0.0 0.0 3.292215 119095 119233 298-301 1.000000 56.3 ndhD-psaC_p4 ndhD-psaC 4 AAGAAAATTAAAGAACCCGCCA ATGGCATGAAACCACTCGAA 22 20 36.4 45.0 56.8 57.8 0.0 0.0 3.412941 119095 119233 247-250 1.000000 55.9 ndhD-psaC_p5 ndhD-psaC 5 AGAAAATTAAAGAACCCGCCAA ATGGCATGAAACCACTCGAA 22 20 36.4 45.0 56.8 57.8 0.0 0.0 3.412941 119095 119233 246-249 1.000000 55.9